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How to fix Relion Prime Error 04?

Alton Alexander
By Alton AlexanderUpdated on June 4th, 2022

There are a few reasons why the relion prime error code 04 might occur. One reason could be that the user has not logged in with the correct credentials. Another reason could be that the user's account has been locked or suspended. Lastly, this error code could also indicate that the user's account has been deleted.

Users typically search for a solution by asking about:

  • relion prime error code 04
  • relion prime error 04

1. Check that the input parameters are valid

The steps to Check that the input parameters are valid in order to fix relion prime error 04 are as follows:

  1. Check that the input parameters are in the correct format.
  2. Check that the input parameters are not blank.
  3. Check that the input parameters are not null.
  4. Check that the input parameters are not empty.

2. Check that the input images are valid

  1. Open the input images in a photo editor.
  2. Check the alignment of the images. If they are not aligned properly, fix the alignment using the tools in the photo editor.
  3. Check the brightness, contrast, and color of the images. If they are not correct, adjust the brightness, contrast, and color using the tools in the photo editor.
  4. Check the resolution of the images. If they are not high enough resolution, resize the images using the tools in the photo editor.

3. Check that the output directory exists and is writable

1.Check if the output directory exists by using the ls -l command If the output directory does not exist, then create it by using the mkdir command. 2.Make sure the output directory is writable by using the chmod command. 3.If the output directory is writable, then fix the relion prime error 04.

4. Check that the number of particles is greater than zero

The first step is to determine the number of particles in the sample. To do this, you need to perform a particle count. The particle count can be done using a variety of methods, including optical or electronic particle counters. The second step is to determine if the number of particles is greater than zero. This can be done using a variety of methods, including the particle size distribution, the mean particle size, or the median particle size. If the number of particles is greater than zero, then the relion prime error 04 is likely fixable.

If the answers above didn't work then you should also try:

  1. Check that the number of classes is greater than one
  2. Check that the number of iterations is greater than zero
  3. Check that the pixel size is greater than zero
  4. Check that the box size is greater than zero
  5. Check that the particle diameter is greater than zero
  6. Check that the angular sampling is greater than zero